This strategy implements BayesHammer ( Nikolenko et al. The genome was assembled employing the assembly service “auto” available in PATRIC (Pathosystems Resource Integration Center Wattam et al. The quality was checked with the program FastQC v0.11.5 ( Andrews 2010). Sequencing resulted in 22,196,922 reads, with length varying from 32 to 151 bases, which comprised a total of 3,351,735, 222 bases and represented 849-fold genome coverage. The sequence data were generated with an Illumina NextSeq-500 using the run kit Illumina NextSeq 500/550 High Output Kit v2. DNA extraction was done according to the method described by Lee et al. Materials and Methods Isolation and DNA Extractionīacillus velezensis UFLA258 was isolated from a soil sample collected in a cotton ( Gossypium hirsutum L.) field in Mato Grosso province, Brazil. Although closely related, they are distinct species with many commonalities and minor differences. Additionally, a taxonomic re-evaluation of the clade B. velezensis– B. amyloliquefaciens was performed. In this study, we sequenced the genome of strain UFLA258 and compared it with all genomes of closely related species. Strain UFLA258 of B. velezensis was isolated from soil around the roots of healthy cotton plants and shown to have potential to control plant pathogens ( Medeiros et al. Although all these species were reclassified as B. velezensis, this information still needs to be integrated into a well-organized resource. plantarum, Bacillus methylotrophicus, and Bacillus oryzicola ( Wang et al. Subsequently, B. velezensis was found to be a heterotypic synonym of B. amyloliquefaciens subsp. Initially, B. velezensis was shown to be closely related to Bacillus subtilis and Bacillus amyloliquefaciens ( Ruiz-García et al. Some of these compounds are active against plant pathogens and/or induce systemic resistance in plants, conferring an adaptive advantage in specific ecological niches ( Mukherjee and Das 2005 Ruiz-García et al. This species synthesizes several types of lipopeptides as products of secondary metabolism. 2005), and since then its biopesticide potential has been unequivocally shown ( Cai et al. 2017).īacillus velezensis was originally described in 2005 ( Ruiz-García et al. The potential of Bacillus isolates for commercial development is enhanced by their fast growth rate and resistance to adverse environmental conditions ( Shafi et al. Members of the genus Bacillus have been used for this purpose due to their ability to produce a large number of biologically active molecules with growth -promoting activity and inhibitory effects against plant pathogens ( Fira et al. Numerous microorganisms have been successfully developed as biopesticides at the commercial level ( Shafi et al. This genomic analysis expands our knowledge about the closely related species, B. velezensis, B. amyloliquefaciens, and B. siamensis, with emphasis on their taxonomical status.īiological control, Bacillus spp, comparative genomics, CRISPr/Cas, secondary metabolites Introduction Although these species are phylogenetically close, the combined analyses of several genomic characteristics, such as the presence of biosynthetic genes encoding secondary metabolites, CRISPr/Cas arrays, Average Nucleotide Identity and Digital DNA–DNA Hybridization, and other information on the strains, including isolation source, allowed their unequivocal classification. In total, 115 genomes were analyzed and taxonomically classified as follows: 105 were B. velezensis, 9 were B. amyloliquefaciens, and 1 was Bacillus siamensis. Analyses based on Average Nucleotide Identity and Digital DNA–DNA Hybridization and a phylogeny with complete sequences of the rpoB gene confirmed that 19 strains deposited in the database as Bacillus amyloliquefaciens were in fact B. velezensis. It contained 3,949 genes encoding proteins and 27 RNA genes. The genome of B. velezensis UFLA258 consisted of a single circular chromosome of 3.95 Mb in length, with a mean GC content of 46.69%. With a comparative genomics approach, in silico analyses of all complete genomes of B. velezensis and closely related species available in the database were performed.
#Quality control biocontrol agents full
In this study, the full genome sequence of Bacillus velezensis strain UFLA258, a biological control agent of plant pathogens was obtained, assembled, and annotated.